【摘要】 进一步探讨小肠移植（ＳＨＴ）排斥反应的机理。方法：用分子生物学方法对小鼠ＳＢＴ术后脾组织中ＣＤ_２３ｍＲＮＡ的转录水平进行动态观察，同期对移植小肠行组织病理学检查，对不同的免疫抑制方案进行评估。结果：ＣＤ_２３ｍＲＮＡ转录水平在同系移植组术后第１天明显升高，术后第５天开始下降，而异系移植组始终保持较高水平。雷公藤多甙（Ｔ_Ⅱ）联合小剂量ＣｓＡ抑制移植术后免疫排斥反应的效果明显优于小剂量ＣｓＡ组，与全量ＣｓＡ疗效相同。结论：ＣＤ_２３的基因转录水平对术后排斥反应和感染的诊断有重要意义。急性排斥反应不仅有细胞免疫，同时也有体液免疫参与，Ｔ_Ⅱ与ＣｓＡ联合应用对抑制小肠移植后排斥反应具有较好的疗效。更多还原

【Abstract】 Purpose: To further research on the mechanism of the rejection of small bowel transplantation(SBT). Methods: The transcription level of CD23 coding genes in postoperative spleens was dynamically assayed. Simultaneously, the pathological evidence of the grafts was studied and different immunosuppresive therapeutic methods were evaluated. Results: The results show that CD23 mRNA was evidently increased at POD 1 and was reduced at POD 5, 7 in groupA, however it was maintained at high level from POD 1 to POD 7 in group B. The effect on inhibiting the episode of rejection of total glycosides of tripterygium wilfordii(TⅡ)combined with partial dosage of CsA was superior to using partial dosage of on only and almost equal to the full dosage of CsA. Conclusion: It indexed that the transcription level of CD23 coding genes had important significance in diagnosing rejective reaction and infection. Acute rejective reaction included not only cell immunity but also humoral immunity. The using of TⅡ combined with partial dosage of on could inhibit acute rejective reaction after small bowel transplantation.更多还原