【摘要】 研究干扰素及干扰素诱导蛋白的基因表达在脑发育中的变化。（１）利用基因工程技术克隆了诱导蛋白１－８家族基因，主要步骤为自未经干扰素处理的胎脑中提取总ＲＮＡ，经Ｏｌｉｇｏ（ｄＴ）－纤维素拉分离纯化出ｐｏｌｙ（Ａ）~＋ＲＮＡ。（２）用ＲＴ－ＰＣＲ法扩增出干扰素诱导的１－８基因家族成员保守区的ｃＤＮＡ片段，即已知的３个成员１－８Ｕ、１－８Ｄ和９－２７的同源序列。将此扩增出的 ＣＤＮＡ片段克隆入ｐＵＣ１８的ＳｍａＩ和 ＥｃｏＲＩ位点之间得重组质粒。用双脱氧末端终止法测定了插入片段的全部核苷酸顺序，证明其长度为１８６ ｂｐ，与家族中３个功能性成员基因１－８Ｕ、１－８Ｄ和９－２７基因相应片段的同源性分别为１００％、９８％和９６．７％。深入的研究工作有待继续。更多还原

【Abstract】 Total RNA from fetal brain tissue was extracted with the acid guanidinium thiocyanate-phenol-chloroform (AGPC) single-step method. Poly(A)~+ RNA was isolated and purified through packed column containing oligo-(dT) cellulose. The consensus sequence of interferon-inducible 1-8 gene family was amplified by reverse transcription-polymerase chain reaction (RT-PCR) method with the template of fetal brain poly(A)~ + RNA. The cDNA fragment was cloned into pUC18 plasmid which was digested with Sma I and EcoR I. Nucleotide sequence of the cDNA fragment was determined by dideoxy- nucleotide chain-termination method. The sequence is in accordance with there reported by other authors.更多还原