【摘要】 进一步探讨小肠移植（ＳＢＴ）排斥反应机理。方法：用分子生物学方法对小鼠ＳＢＴ术后牌组织中白细胞介素－４（Ⅱ－４）的基因转录水平进行动态观察，对移植小肠行同期组织病理学检查，对不同的免疫抑制方案进行评估。结果：同系移植组术后１周内几乎无ＩＬ４基因转录，异系移植组术后第３天ＩＬ－４基因转录水平开始升高，术后第５、７天达高峰。而组织病理于术后第７天才开始出现排斥反应。雷公藤多甙（Ｔ_Ⅱ）联合小剂量ＣｓＡ抑制移植术后免疫排斥反应的效果明显优于小剂量ＣｓＡ，与全量ＣｓＡ疗效相同。结论：ＩＬ－４基因转录水平可作为诊断早期排斥反应的标志物；雷公藤多甙联合小剂量治疗ＣｓＡ可抑制ＩＬ－４基因转录。更多还原

【Abstract】 For the research on the mechanism of rejection of small bowel transplantation(SBT), the transcription level of IL-4 coding genes in postoperative spleen was dynamically assayed. Simultaneously, the pathological evidence of the grafts was studied and different immunosuppressive therapeutic methods were evaluated. The results showed that few transcription of IL-4 mRNA was found within the first week after transplantation in group A. The transcription level of IL-4 mRNA began to increase from postoperative day(POD)3 and reached high peak on POd 5 and the same high transcription level accompanied with obvious pathological evidence of rejection were found on POD 7 in group B. The effect on inhibiting the episode of rejection of total glycosides of tripterygium wilfordii(T_Ⅱ )combined with partial dosage of cyclosporin A(CsA)was superior to using partial dosage of CsA only and almost equal to the full dosage of CsA. It indicated that the transcription level of IL-4 coding genes can be taken as the maker for diagnosing early rejective reaction. The using of T_Ⅱ combined with partial dosage of CsA can inhibit the transcription of IL-4.更多还原