【摘要】 使用逆转录聚合酶链反应（ＲＴ－ＰＣＲ）检测恶性黑素瘤患者外周血中酪氨酸酶ｍＲＮＡ，并与健康供血者和其它皮肤恶性肿瘤患者对照。结果：６８例恶性黑素瘤患者，临床Ⅰ期和Ⅱ期酪氨酸酶ｍＲＮＡ的ＲＴ－ＰＣＲ阳性率分别为６．５％和１３．３％，Ⅲ期为４２．９％，Ⅳ期（即有远距离转移者）为８７．５％（Ｐ＜０．００１）。说明外周血中酪氨酸酶ｍＲＮＡ阳性率与临床分期有明显的关系，而与性别、肿瘤发病部位和病理学分类无关（Ｐ＞０．０５）。在健康供血者和其他皮肤恶性肿瘤患者血液中酪氨酸酶ｍＲＮＡ的ＲＴ－ＰＣＲ均为阴性。提示酪氨酸酶ｍＲＮＡ具有特异性。更多还原

【Abstract】 By using reversetranscriptase polymerase chain reaction (RTPCR) tyrosinase mRNA in peripheral blood was determined in the patients with malignant melanoma and compared with that in healthy subjects and patients with other malignant tumor in skin Blood samples of 68 patients with malignant melanoma in different stages of disease were collected RTPCR positive rate for tyrosinase mRNA in blood were 65%, 133%, 429% and 875% in the patients with stages I to , respectively. There was a statistically significant correlation between positive RTPCR products for tyrosinase mRNA in peripheral blood and clinical stage(P<0001) In control, RTPCR products for tyrosinase mRNA were negative in all of healthy subjects or patients with other malignant tumor in skin These results demonstrate that tyrosinase mRNA is specific更多还原