【摘要】 为观察穿心莲有效成分（ＡＰＩ０１３４）对凝血酶作用下血小板活化的抑制作用，将家兔２４只随机分为ＡＰＩ０１３４（Ａ）组（ｎ＝９），噻氯匹定（Ｔ）组（ｎ＝８）和对照组（ｎ＝７），治疗１周，然后检测凝血酶作用下血小板内ｃＡＭＰ、ｃＧＭＰ和胞浆游离钙离子浓度，以及血小板超微结构。结果表明ｃＡＭＰ水平在Ａ、Ｔ两组显著高于Ｃ组（Ｐ＜０．０５），而胞浆游离钙离子浓度明显低于Ｃ组（Ｐ＜０．０１），超微结构表明Ａ组和Ｔ组血小板脱颗粒现象较Ｃ组明显减轻。在Ａ和Ｔ组之间各项参数均无统计学差异。结论：ＡＰＩ可抑制凝血酶诱导的血小板活化反应更多还原

【Abstract】 In order to observe the inhibitory effect of Andrographis Paniculata Isolate 0134(API 0134 ,API) on the anti thrombin induced platelet activation, 24 domestic rabbits were randomly divided into API pretreated group ( n= 9), ticlopidine (T) pretreated group ( n= 8), and control (C) group ( n= 7). The rabbits received the treatment for 1 week. After induction with thrombin following parameters were measured: cAMP, cGMP and concentration of cytoplasmic free Ca 2+ . Ultrastructural changes of platelets were also examined. cAMP level was markedly higher in API and T groups than in C group ( P <0.05), while the concentration of free Ca 2+ in both API and T groups was markedly lower than in C group ( P <0.01). The ultrastructural changes of platelets showed the degranulation in API and T groups was much milder than in C group. API may inhibit thrombin induced activation of platelets.更多还原