【摘要】 目的：采用多重ＰＣＲ法检测喉癌及癌旁组织中Ｐ１６ 基因纯合缺失和异常甲基化。方法：选用二对引物分别扩增Ｐ１６ 基因外显子１ 和２，分析有无纯合缺失，对未检出缺失的样品用甲基化敏感内切酶Ｓｍ ａＩ消化后，再扩增外显子１，分析有无Ｐ１６ 基因异常甲基化。结果：３９ 例喉癌组织标本中９ 例标本有Ｐ１６ 基因纯合缺失，缺失率为２３．０８％ （９／３９）。３０ 例未检测到Ｐ１６ 基因缺失的喉癌组织标本，有４ 例检出异常甲基化，检出率为１３．３３％ （４／３０）。３９ 例癌旁组织标本均未检测到Ｐ１６基因缺失或异常甲基化。结论：采用多重ＰＣＲ法可检测出Ｐ１６ 基因常见的纯合缺失和异常甲基化失活。研究表明，Ｐ１６ 基因失活可能在喉癌的发生发展中起重要作用更多还原

【Abstract】 Objective:By using multiple PCR to detect homozygous deletions and aberrant DNA methylation of MST1/P16 gene in laryngeal cancer and tumor-adjacent tissue specimens. Methods: Using two pair of primes to amplify P16 gene for analysing homozygous deletion in exon 1 and exon 2. Samples which had not found homozygous deletion were digested by using a restriction endonuclease SmaI ,then amplified exon 1 and analysed aberrant DNA methylation. Results: 9 cases of laryngeal cancer revealed homozygous deletion of P16 gene, the deletion rate was 23.08%(9/39) among the 30 cases laryngeal cancer which hadn’t revealed gene deletion,four cases of abnormal methylation were detected, the rate of aberrant methylation was 13.33%(4/30). No homozygous deletion and aberrant methylation were revealed in tumor-adjacent tissue.Conclusion: The homozygous deletion and aberrant DNA methylation of P16 gene can be deteced by using mutiple PCR. Inactivation of P16 gene due to homozygous deletion or aberrant methylation are possibly an importent factor in laryngeal cancer development.更多还原