【摘要】 目的：测定急性肾小球肾炎患儿血和尿中一氧化氮和环鸟苷酸，以期了解一氧化氮在急性肾炎发病过程中的变化。　方法：分别采用分光光度和放射性免疫分析的方法测定了１７ 例急性肾小球肾炎急性期患儿血和尿中的ＮＯ－２ ／ ＮＯ－３ 和ｃＧＭＰ，并对６ 例患儿进行了急性期和恢复期的动态观察。　结果：１７ 例急性期患儿血中ＮＯ 明显升高（６９－２ ±１５－７ μｍ ｏｌ／Ｌ ｖｓ ３１－３ ±４－６ μｍ ｏｌ／Ｌ，Ｐ＜ ０－０５） ，尿中ｃＧＭＰ 降低（３６６－１ ±３９－０ｐ ｍｏｌ／ ｍ ｇ ｖｓ６３７－６ ±７８－６ ｐｍ ｏｌ／ ｍ ｇ ，Ｐ＜ ０－０５） ，但尿中ＮＯ－２ ／ ＮＯ－３ 和血ｃＧＭＰ 与正常对照组差异不显著。动态观察的６ 例患儿急性期尿ＮＯ 和ｃＧＭＰ 明显低于恢复期（５９０－８ ±１４１－４ ｎ ｍｏｌ／ ｍ ｇ ｖｓ １６０５－６ ±１４９－２ ｎ ｍ ｏｌ／ ｍ ｇ ，Ｐ＜ ０－０５） ；（２８３－４ ±６１－７ ｐｍ ｏｌ／ ｍ ｇ ｖｓ １０８３－３ ±３３６－９ ｐ ｍｏｌ／ ｍｇ ，Ｐ＜ ０－０５） 。同时急性期血中ＮＯ 明显高于恢复期（１０５－９ ±２７－３ μｍｏｌ／ Ｌｖｓ ４６－５ ±１０－１ μｍ ｏｌ／Ｌ，Ｐ＜ ０－０５） 。　结论：急性肾炎患儿在?更多还原

【Abstract】 To investigate the changes of urinary and plasma NO and cGMP in children with acute glomerulonephritis(AGN)and its significance in the pathogenesis of AGN. METHODOLOGY Seventeen children with AGN and eighteen healthy children were involved in this study.Urinary and plasma NO 2/NO 3 were determined with biochemical assays.The levels of urinary and plasma cGMP were determined with radioimmunoassay.In six of the seventeen AGN patients,dynamic changes of urinary and plasma NO and cGMP were investigated. RESULTS As compared to the controls,the mean plasma NO 2/NO 3 was found significantly increased (69 2±15 7 μmol/L vs 31 3±4 6 μmol/L, P <0 05)and urinary cGMP decreased (366 1±39 0 pmol/L vs 637 6±78 6 pmol/L, P <0 05)in the 17 AGN children.No significant difference in urinary NO 2/NO 3 and plasma cGMP was found between the AGN patients and the control.In the follow up study of the six AGN patients,urinary NO 2/NO 3 and cGMP were found significantly decreased at the acute phase as compared to those at the recovery phase(590 8±141 4 vs 1605 6±149 2 nmol/mg;283 4±61 7 vs 1083 3±336 9 pmol/mg, P <0 05),while plasma NO 2/NO 3 was significantly increased at the acute phase as compared to those at the recovery phase(105 9±27 3 vs 46 5±10 1 μmol/L, P <0 05). CONCLUSION Remarkable changes of urinary NO 2/NO 3 and cGMP occur in AGN children.NO might play an important role in the pathogenesis of AGN.更多还原