【摘要】 采用聚合酶链反应技术（PCR）检测不育双方生殖道沙眼衣原体（CT）及溶脲脲原体（UU）DNA，酶标双抗体夹心法测定不育患者精浆TNF－α、IL－6含量。发现感染在不育双方呈平行性表达（P＞0．05），并导致不育患者精浆中TNF－α、IL－6明显增高（P＜0．05）。感染致精浆细胞因子的升高可能影响精子的密度、活动率、液化程度，并参与生殖器官的炎性损伤。提示精浆炎性细胞因子及CT、UU感染参与男女不育的发病过程。更多还原

【Abstract】 An examination was made of IL-6 and TNF-α in seminal plasma and DNA of chlamydial trachomatis (CT) and U. urealyticum in genital tract of infertile couple, using ELISA and PCR respectively. The results showed there was an increase of TNF-α and IL-6 and parallel relation between CT and UU infection and TNF-α and IL-6 experssion. Infection and cytokine expression not only affect sperm density,motility rate and liquefiaction but also participated in inflammatory lesion of genital organs. These suggested cytokines and CT,UU infection participated in infertile process.更多还原