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DNA相对含量与抗辐射菌辐射抗性间的关系

Relationship between the Relative Content of DNA and Protein and the Radioresistance in Deinococcus Radiodurans

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【作者】 段蓉芳; 杜泽吉;

【Author】 Duan ROnedng, Du Zeji (Department of Preventive Medicine, Suzhou Medical College, 215007)

【机构】 苏州医学院预防医学系!215007; 苏州医学院放射医学系;

【摘要】 通过改变抗辐射菌的培养条件,结合细胞内DNA和蛋白质测定,在细胞水平上阐明了抗辐射菌的辐射抗性与其DNA相对含量无密切关系,而与其生长状态密切相关,改变细胞处于指数生长状态的培养条件,都能增加其对辐射的抗性。并对可能的机理加以讨论。

【Abstract】 Radioresistant bacterium Deinococcus radiodurans (D. radiodurans) prossesses a prominent ability to resist ionizingradiation and other DNA - darnaging agents. The amount of DNA relative to protein has been considered to reflect the ra-dioresistance of a bacterium. In this study, D. radiodurans was cultured exp0nentially and under conditions that altersuch growth. The resuIts showed that disruptions of exponential growth increased Gamma - ray resistance regardless of thechange in DNA - protein ratio. The results suggested that at the celIular levei the glntvth stages are responsible for the ra-dioresistance of D. radiodurans.

  • 【分类号】R14
  • 【被引频次】3
  • 【下载频次】59
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