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基因工程人干扰素-α2a抗柯萨奇病毒的实验研究

The Inhibition Effect on the Tumor Cells Growth by the Recombinant interferon - α2a

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【作者】 缪竞诚杨吉成盛伟华吕海涛李丽娥董宁征

【Author】 Liao Jingcheng, et al (Biotechnological institute of Suzhou Medical College,215007)

【机构】 苏州医学院生物技术研究所!215007

【摘要】 采用微量细胞病变抑制法在Wish细胞上研究了IFN-α2a抗柯萨奇病毒的作用。结果表明:万复洛和罗扰素在Wish细胞上500IU/ml可以抗3lo4,50IU/ml可以抗2log4,5IU/ml可以抗1log4柯萨奇病毒的效应。

【Abstract】 Purpose: To study the anti-tumor effect of various subtypes of the recombinant IFNa from different manufactories.Methods: MTT assay was applied to Investigate the inhibition of various tumor cells growth by IFNa. Results: The groWthof SHG -44, HOS-8603, HL-60, K562 and Ran cells were all inhibited by various IFNα2a. at dose 10, 000IU/ml, l,000IU/all and 100lU/ml with the according inhibitory rate about 20%, 10% and 5 %. Conclusion: Both the importedand domestic IFNα2a could produce remarkable inhibition effect on these five kinds of tumor cells with the inhibitory rateswere similar.

  • 【文献出处】 苏州医学院学报 ,ACTA ACADEMIAE MEDICINAE SUZHOU , 编辑部邮箱 ,1999年04期
  • 【分类号】R965
  • 【下载频次】24
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