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甘蓝夜蛾高毒力Bt菌株的筛选

The Screen of Bacillus thuringiensis Strains of High Toxicity on Mamestra brassicae(L.)

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【作者】 樊东; 李国勋; 赵奎军; 韩岚岚; 王君毅;

【Author】 FAN Dong; LI Guo-xun; ZHAO Kui-jun; et al.(Department of Plant Protection, Northeast Agricultural University, Harbin 150030)

【机构】 东北农业大学植物保护系!黑龙江哈尔滨; 150030;

【摘要】 东北农业大学昆虫教研室从黑龙江地区病死鳞翅目昆虫体内分离出苏云金杆菌(Bacillusthuringiensis,Bt)菌株74株,随后以甘蓝夜蛾为供试昆虫对这些菌株的毒力进行了测定,结果表明,很多菌株对甘蓝夜蛾的初孵幼虫均具有一定的毒力,经过初筛、复筛,试验选出了毒力相对较高的5个菌株,当菌液浓度在8×10~7个芽孢·mL(-1)时,甘蓝夜饿死亡率分别达到80%~95%,Lc50分别为43,63,68,710,1778倍液。

【Abstract】 74 Bacillus thuringiensis (Bt) strains were isolated from corpses of Lepidopterous insects. The strains were used to conduct bioassay compared with their toxicties on cabbage armyworm Mamestra brasica(L.). The results showed that M. brassicae larvae were sensitive to many of the strains. Five Bt strains of high toxicity were also screened according to the bioassay. The mortality of M. brassicae larvae treated with the 5 strains at concemration of 8×107 spores. mL-1 was 80% ~ 95%. The Lc50 of the 5 strains to M. brassicae larvae were 43, 63, 68, 710and 1778 respectively.

【关键词】 甘蓝夜蛾; 苏云金杆菌; 毒力;
【Key words】 Mamestra brassicae; Bacillus thuringiensis; toxicity;
  • 【文献出处】 沈阳农业大学学报 ,JOURNAL OF SHENYANG AGRICULTURAL UNIVERSITY , 编辑部邮箱 ,1999年03期
  • 【分类号】S476.1
  • 【被引频次】6
  • 【下载频次】82
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