【摘要】 目的：建立舌癌远隔脏器转移细胞系。方法：采用癌细胞尾静脉注射法、细胞培养法从裸鼠体内获取人舌癌Tca8113细胞的远隔脏器转移灶细胞系，用染色体显示、细胞形态及细胞致瘤性观察证明其性质，用细胞计数法，流式细胞仪检测细胞增殖速度。结果：20只受试探鼠中1只神经症状明显，取脑组织行细胞培养获得细胞系，传代150次以上，维持培养3年，生长稳定，倍增时间为20．1h，S期细胞占29．0％，染色体众数为66，保持人类染色体形态，细胞形态与Tca8113相似，所致棵鼠皮下肿瘤为典型鳞状上皮癌，将该细胞系命名为Tb。结论：Tb细胞系是八舌癌Tca8113细胞在裸鼠体内的脑转移细胞系。更多还原

【Abstract】 To establish a metastatic cell line from distant organ metastasis usingtongue cancer Tca8113 cells in unde mice.Method: Tail vein injection f cancer cells andcell culture technic were employed t inducemetastasis in distant organ and to obtain cell linefrom the organ. Karytype analysis and histopathological observatin were used t study thebiological nature of the cells. Cell counting andflow cytometry were used to study the cellgrowth. Results: Suspected braln metastasis wasobserved in 1/20 nude mice. Cells derived fromthe brain tissue had been cultured for 3 years andtransferred for more than 150 passages. Thecells were proved to be of squamus cell carcino-ma frm human being by the mrphlogy,histopatholgy and karyotype of the cells. Thepopulation doubling tirne and s-phase cell uf thecells were 2. 1 h and 29. 7% respectlvely. Thecell line was named Tb. Cnclusin: Tb is ametastatic cell line of brain metastasis f humantongue cancer Tca8113 cells in nude muse.更多还原