【摘要】 目的 :探索快速、有效、简便的检测食管癌 P5 3基因突变的方法 ,以利于食管癌的早期诊断和研究。方法 :用银染 PCR- SSCP法检测本院 63例食管切除标本的 P5 3基因突变 (选突变多发位点 5、6、7、8外显子 )。结果 :食管癌 P5 3基因突变发生于第 5、6、7、8外显子 ,总突变率 68% ,鳞癌、腺癌、未分化癌中 P5 3突变率差异不显著 ;鳞状上皮不典型增生中 P5 3突变率为 61.5 %。结论 :P5 3基因突变可作为食管癌早期诊断的一个重要分子生物学指标更多还原

【Abstract】 Objective:To establish a simple and efficient method to detect the expression of P53 gene mutation in esophageal carcinoma for its early diagnosis.Methods:Single strand conformation polymorphism(PCR SSCP)combined with silver staining as a method was established to screen the expression of P53 gene mutation(the exons 5,6,7,8)in 63 esophageal carcinomas.Results:The expression of P53 gene mutation in esophageal carcinomas frequently happened at the exons 5,6,7,8,mutation rate 68%(34 of 50).There is no significant difference among squamous cell carcinoma,adenocarcinoma and undifferentiated carcinoma.In addition,P53 gene mutation rate is 61.5%(8 of 13)in the atypical hyperplasia of squamous epithelium.Conclusion:The expression of P53 gene mutation may be used as a valuable index of molecular biology;PCR SSCP argentation is a reasonable method for the early diagnosis of esophageal carcinoma.更多还原