【摘要】 目的：建立选择性好、基本无干扰的血清钙偶氮胂Ⅲ显色EDTA褪色测定法。方法：在碱性条件下，偶氮胂Ⅲ与钙络合显色，加入EDTA使钙解离，测定吸光度的下降来分析血清钙。结果：线性范围为0～5mmol／L；批内和批间平均变异分别为1．1％和2.0％，平均回收率为100．4％，在血清胆红素高达400μmol／L、血红蛋白13g／L、甘油三酯18mmol／L时，均对该法无显著干扰，该法与邻甲酚酞络合酮测定法结果相关性好，r＝0.991。结论：该法选择性好、简便、快速、无干扰，可用于血清钙的常规分析，更适合自动分析。更多还原

【Abstract】 Objective: To create a good-selectivity and no interference method for the determination of calcium in serum by complexing with alkaline Arsenazo Ⅲ and dissociated by EDTA. Methods In the alkaline medium,Arsenazo Ⅲ was used as a coloring reagent to complex with calcium and EDTA was added for dissociating calcium from Arsenazo Ⅲ. Calcium was analyzed by the determination of the absorbent declining volume. Result The lin-earity was from 0 to 5 mmol/L. The average within-run CV and between-run CV were 1. 1% and 2. 0%. The average recovery rate was 100. 4%. The assay showed no interference from as much as 400 μmol/L of bilirubin,13 g/L of hemoglobin and 18 mmol/L of triglyceride. The result from proposed method showed good agreement with result by OCPC procedure,r= 0. 991. Conclusion The method was convenient,rapid and had good-selectiv-ity,no interference. The method was suitable for the routine analysis of calcium in serum,especially for automaticanalysis.更多还原