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绿宝石蔓绿绒离体培养繁殖技术研究
Study of in Vitro Culture Propagation Technique of Philodendron erubescens‘Green Emerald’
【摘要】 绿宝石蔓绿绒离体培养繁殖技术研究结果表明:①冬春两季的茎尖、带芽茎段是理想的外植体材料;②外植体用75% 酒精消毒15 s,再用01% 升汞浸泡5 m in,可达到较好的消毒效果;③最佳分化增殖培养基MS+ BA2 m g/L+ NAA03 m g/L,可使增殖倍数达750;④最佳生根培养基濎濚MS+ NAA05m g/L+ IAA01m g/L+ 蔗糖20g/L+ 活性炭2g/L,达到962% 的瓶苗生根率;⑤组培苗按照最佳移栽程序,可获得9121% 的移栽成活率。
【Abstract】 The study result of in vitro culture propagation technique of Philodendron erubescens ‘Green Emerald’ showed that:① stem apex and stem with buds in winter and spring was ideal explant materials.② disinfecting with 75% alcohol for 15 s,and then soaking with 0 1% corrosive sublimate can reach good effect of disinfectation.③ the best medium for differentiation MS+BA 2 mg/L+NAA 0 3 mg/L makes 7 5 times multiplication.④ the best medium for rooting 1/2 MS+NAA 0 5 mg/L+IAA 0 1 mg/L+20 g/L sucrose+AC 2g/L results in rooting percentage 96 02%.⑤ transplanting of tissue plant can get 91 21% of survival rate under best transplanting procedure.
【Key words】 Philodendron erubescens ‘Green Emerald’; in vitro culture; propagation;
- 【文献出处】 山西林业科技 ,SHANXI FORESTRY SCIENCE AND TECHNOLOGY , 编辑部邮箱 ,1999年03期
- 【分类号】S687.3;S722.37
- 【被引频次】4
- 【下载频次】76