【摘要】 文中对华北落叶松根系多酚氧化酶动力学特性及其同工酶进行分析。结果表明：该酶对邻苯二酚（二元酚）和焦性没食子酸（三元酚）都能氧化，但对前者亲和力较强；ｐＨ７．０时酶活性最强；Ｃｕ２＋提高多酚氧化酶活性；ＥＤＴＡ，Ｈ３ＢＯ３及半胱氨酸可抑制该酶活性；聚丙烯酰胺凝胶电泳将根系多酚氧化酶分出７条同工酶带。更多还原

【Abstract】 This paper studies polyphenoloxidase kinetic properties and isozymes from roots of Larix principis-rupprchtii. Results show that both catechol and 1.2.3-hydroxyphenyl can oxidaze polyphenoloxidase.But the second has a higher affinity to polyphenoloxidase.When pH is 7.0,polyphenoloxidase has the biggest activity.Cu +2 can increase its activity.Polyphenoloxidase of roots was analysed by PAGE,approximately seven bands were detected.更多还原