【摘要】 应用四甲基偶氮唑盐 (MTT)光比色法 ,测定了茶色素及其与重组人肿瘤坏死因子合用对原发性肝癌细胞的细胞毒作用。结果表明 ,茶色素单用对肝癌细胞H740 2细胞无细胞毒作用 ,抑制作用不明显 ;rTNF单用对H740 2细胞的最大抑制率为 35 .1% ,两者合用对H740 2细胞的最大抑制率达 47.2 % ,茶色素能明显增强rTNF对H740 2细胞的细胞毒性作用。更多还原

【Abstract】 The cytotoxic effect of combined administration of tea pigment and recombinant human tumor necrosin factor(rTNF) on primary liver cancer cell was detected by MTT colorimetric assay. When tea pigment or rTNF was administered alone, no significant cytotoxic or inhibition effects of tea pigment on liver cancer cell H7402 were observed,and the maximal inhibition rate of rTNF on the cell was 35.1%.However, the maximal inhibition rate of combined administration of the 2 drugs reached 47.2%. The result showed the the cytotoxic effect of rTNF on H7402 cell could be improved by tea pigment.更多还原