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构建分子标记连锁图谱的图论构图方法

Graphic Theoretical Method for Mapping Molecular Marker Loci

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【作者】 谭远德万春玲周泽阳鲁成向仲怀

【Author】 Tan Yuande; Wan Chunling; Zhou Zeyang; Lu Cheng; Xiang Zhonghuai ( Life College, Human Normal University, Changsha 410081) (The Key Sericultural Laboratory of Agricultural Ministry, Southwest Agricultural University Chongqing 400716) ( Life College,

【机构】 湖南师范大学生命科学学院!长沙 410081农业部蚕桑重点开放实验室 西南农业大学蚕丝学院 重庆 400716南京师范大学生命科学学院!南京 210097农业部蚕桑重点开放实验室!西南农业大学蚕丝学院 重庆 400716农业部蚕桑重点开放实验室!西南农业大学蚕丝学院 重庆 40071

【摘要】 结合统计学、遗传学和图论原理,建立了一种新的分子标记连锁图谱构建的方法,即图论构图法.应用这种方法,从77个家蚕RAPD标记位点中构建了7个标记位点的两个连锁群.与MAPMARKER程序所构建的结果相比表明,图论构图法具有更为可靠的构图效果.

【Abstract】 Construction of molecular marker-based linkage map has become one of the hot points in molecular biology. At the same time, statistic methods and softwares for mapping molecular marker loci have been developed. As a study of methodology, a new approach, graphic theoretcial method, was developed on the basis of combination of statistics, genetics and graphic theory in this paper. Two linkage groups including 7 RAPD loci were mapped from 77 RAPD markers in silkworm (Bombyxmori. L.) by our approach. As compared with the mapping these 77 RAPD markers by MAPMARKER program, the result shows that our approach of graphic theory is more confident in mapping molecular marker loci.

【基金】 国家自然科学基金!(39870568);国家自然科学基金!(3970730)
  • 【文献出处】 生物数学学报 ,JOURNAL OF BIOMATHEMATICS , 编辑部邮箱 ,1999年03期
  • 【分类号】Q343
  • 【被引频次】10
  • 【下载频次】220
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