节点文献

Isoverbascoside对HL-60细胞的诱导分化和细胞毒作用

INDUCED-DIFFERENTIATION AND CYTOTOXICITY OF ISOVERBASCOSIDE ON HL-60 CELLS

  • 推荐 CAJ下载
  • PDF下载
  • 不支持迅雷等下载工具,请取消加速工具后下载。

【作者】 王天叫杨善民黄宗平李祺福李庆阁周红郑荣梁贾忠健

【Author】 WANG Tian Jiao YANG Shan Min HUANG Zong Ping LI Qi Fu LI QingGe ZHOU Hong ZHENG Rong Liang JIA Zhong Jian(Cancer Research Center , Department of Biology , Xiamen University , Xiamen , 361005; Department of Biology , Lanzhou University, Lanzhou, 730000)

【机构】 厦门大学抗癌研究中心厦门大学生物系兰州大学生物系兰州大学生物系 厦门 361005厦门 361005兰州 730000兰州 730000

【摘要】 不同时间、不同浓度的isoverbascoside体外处理HL-60细胞,以形态改变(光镜和透射电镜观察)、功能分化(化学发光检测吞噬能力)、恶性度降低(裸鼠成瘤试验)等指标观察其诱导分化作用;以台盼蓝拒染作用和电镜下形态变化确定其细胞毒作用;用流式细胞术测定其对HL-60细胞周期的影响。20—25μmol/L Isov 1—3天诱导HL-60细胞向粒系方向分化,细胞吞噬能力提高,裸鼠成瘤性降低。30—35μmol/L Isov 2—3天对HL-60细胞有强烈的细胞毒作用。20μmol/L Isov处理12h可引起HL-60细胞的G1期阻滞,在72h时引起HL-60细胞的G2/M期的阻滞。

【Abstract】 HL-60 cells were treated by isoverbascoside with different time and different concentrations in vitro . The differentiation of HL-60 cells was evaluated by light and electron microscopy to observe morphological changes , by chemilumi-nence to detect phagocytosis and by tumorigene-sis in nude mice to determine malignancy . The cytotoxical effect of isoverbascoside on HL-60 cells was examined by trypan blue excluding staining and electron microscopy. The influence of isoverbascoside on cell cycle was measured byflow cytometry. Granular differentiation of HL-60 cells was induced by isoverbascoside at 20-25μmol/L within 1-3 days as the results of morphological changes , enhancement of phagocytosis and decreasing of tumorigenesis . Strong cy-totoxicity was evidenced in HL-60 cells treated by isoverbascoside at 30-35μmol/L. HL-60 cells treated by isoverbascoside at 20μmol/L were delayed at G1 phase at 12 hours and G2/M phase at 72 hours.

【基金】 福建省自然科学基金~~
  • 【文献出处】 实验生物学报 ,Acta Biologiae Experimentalis Sinica , 编辑部邮箱 ,1999年04期
  • 【分类号】R730.5;
  • 【被引频次】1
  • 【下载频次】46
节点文献中: 

本文链接的文献网络图示:

本文的引文网络