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Acetobacter xylinum生产纤维素的最适培养基成分
INVESTIGATION OF OPTIMAL MEDIUM COMPOSITION FOR MICROBIAL CELLULOSE PRODUCTION BY ACTEBACTER XYLINUM
【摘要】 探索出胶醋杆菌在摇瓶培养最适培养基成分为蛋白胨浓度1.0%、酵母膏0.5%、葡萄糖浓度2.0%、柠檬酸0.115%、乙醇1%、Na2HPO40.5%,pH6.0,160r/min的条件下进行摇瓶培养,细菌纤维素最大产量为7.55g/l。
【Abstract】 complex medium was developed for the production of microbial cellulose by Acetobacter xylinum. The optimum concentration of each nutrient for the production of microbial cellulose was determined to be peptone 1%, yeast extract 0.5%, glucose 2%, Na 2HPO 4 1%, citric acid 0.115%, ethanol 1%. The maximal yield of cellulose productionis 7.55g/l at shaking speed 160 r/min, pH6.0. Cell growth and the cellulose production in the developed medium under shakingflask culture was also investigated.
【关键词】 胶醋杆菌;
细菌纤维素;
最大产量;
摇瓶培养;
【Key words】 Acetobacter xylinum; bacterial cellulose; maximal yield; shaking-flask culture;
【Key words】 Acetobacter xylinum; bacterial cellulose; maximal yield; shaking-flask culture;
- 【文献出处】 生物技术 ,BIOTECHNOLOGY , 编辑部邮箱 ,1999年03期
- 【分类号】TQ924
- 【被引频次】27
- 【下载频次】144