【摘要】 为了阐明甲状旁腺素（ｐａｒａｔｈｙｒｏｉｄｈｏｒｍｏｎｅ，ＰＴＨ）、胰岛素样生长因子－Ⅰ（ｉｎｓｕｌｉｎ－ｌｉｋｅｇｒｏｗｔｈｆａｃｔｏｒ－Ⅰ，ＩＧＦ－Ⅰ）在促成骨样细胞ＲＯＳ１７／２．８增殖的同时对其分化状况的影响，研究了ＰＴＨ、ＩＧＦ－Ⅰ对骨特征性标志蛋白碱性磷酸酶（ａｌｋａｌｉｎｅｐｈｏｓｐｈａｔａｓｅ，ＡＬＰ）活性、骨钙素（ｏｓｔｅｏｃａｌ－ｃｉｎ）ｍＲＮＡ表达及骨胶原蛋白（ｃｏｌａｇｅｎ）合成的影响．结果表明，ＰＴＨ、ＩＧＦ－Ⅰ均可抑制ＡＬＰ活性，ＰＴＨ的抑制作用强于ＩＧＦ－Ⅰ，抑制作用具有时间剂量依赖性；ＰＴＨ、ＩＧＦ－Ⅰ均可诱导骨钙素ｍＲＮＡ表达增加；ＰＴＨ能够促进３Ｈ－脯氨酸参入新生小鼠颅骨组织，既ＰＴＨ能够骨胶原蛋白合成增加．这些结果提示，ＰＴＨ、ＩＧＦ－Ⅰ在促成骨样细胞ＲＯＳ１７／２．８增殖的同时也促进其分化，表明在成骨细胞的生发成熟过程中，增殖、分化现象相辅相成，相伴而行．更多还原

【Abstract】 Parathyroid hormone(PTH)plays an important role in regulation of calcium and phosphorus homeostasis.Recently it has been demonstrated that PTH can also enhance hone anabolism.In osteoblast like ROS 17/2 8 cells the proliferative effect of PTH has already been observed.This report describes the differentiative effects of PTH(10 -9 and 10 -10 mol/L)that down regulated alkaline phosphates activity,increased the expression of osteocalcin mRNA,and enhanced the incorporation of 3 H proline into collagen.Similar results were also demonstrated in IGF Ⅰ treated ROS 17/2 8 cells.Alkaline phosphatase,osteocalcin and collagen are three main marker proteins of differentiated osteoblast.It means that PTH and IGF Ⅰ can both regulate proliferation and differentiation at different stages of cell growth in osbteoblast like ROS 17/2 8 cells.更多还原