【摘要】 采用紫外线对泥鳅和大鳞副泥鳅卵雌性原核进行干法灭活以及在合成卵巢液、TC-199溶液、Ringer氏溶液和Holtfreter氏溶液中灭活,结果表明:当辐射剂量为90-180mJ/cm~2时,卵子在四种溶液中的单倍体诱导率均较高。合成卵巢液组的最佳辐射剂量为120-180mJ/cm~2;TC-199和Ringer氏溶液组的最佳辐射剂量为120mJ/cm~2;原液使用Holtfreter氏溶液效果欠佳,干法灭活效果最差。在上述各辐射剂量下,畸形苗经染色体组鉴定92％为雄核发育单倍体。综合评价,灭活效果依次是:人工合成卵巢液>TC-199溶液>Ringer氏溶液>Holtfreter氏溶液>干法。更多还原

【Abstract】 The genetic inactivation of eggs is one of the key techniques in fish anadrogenesis. The present paper deals with ultraviolet (UV) rays irradiating for genetic inactivation of loach (Misgurnus Angullicaudatus and Paramisgurnus dabryanus) eggs. Five treatments of irradiation were examined, including irradiating the eggs with solution-free, synthetic o-varian fluid (OF), TC-199 solution, Ringer’s solution, and Holtfreter’s solution. The results showed that high yield of hyploid was induced for the eggs stored in all solutions at total energy of irradiation ranged 90-180mJ/cm2. The rtional dosages of UV irradiation were from 120 to 180mJ/cm2 in OF, 120mJ/cm2 in TC-199 and in Renger’s solution. The inactivating effect of female nucleus was found best when irradiating the eggs stored in OF, then decreased subsequently when the eggs stored in TC-199 solution, Ringer s solution, Holte-freter s solution, and solution-free.更多还原