【摘要】 测定了乙醇在不同温度下的粘度， 比较了乙醇与甲醇的理化性质和作反相高效液相色谱（ Ｒ Ｐ Ｈ Ｐ Ｌ Ｃ）溶剂的特点。用乙醇水作 Ｒ Ｐ Ｈ Ｐ Ｌ Ｃ 流动相测定中药有效成分， 并将测定结果与甲醇水或乙腈水作流动相的测定结果进行比较。研究结果表明， 选择合适的柱温等色谱条件， 乙醇一般可以代替甲醇或乙腈用作 Ｒ Ｐ Ｈ Ｐ Ｌ Ｃ流动相。更多还原

【Abstract】 The viscosity of ethanol water at various temperatures was determined.The physicochemical properties and characters of the solvents used as the mobile phase of reversed phase high performance liquid chromatography(RP HPLC) were compared between methanol and ethanol.The components of several Chinese medicines have been determined by RP HPLC with ethanol water used as mobile phase , and the results were compared with those obtained with methanol water or acetonitrile water. It showed that ethanol can be used as RP HPLC mobile phase by increasing the column temperature moderately .更多还原