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火锅底料中罂粟壳的高效液相色谱法测定
Determination of Pericarpium Papaveris (Yingsuqiao)in Bottom Material of Chafing Dish by High Performance Liquid Chromatography (HPLC)
【摘要】 采用高效液相色谱法,以C18柱为分析柱,V(甲醇):V(25mmol/LKH2PO4)=90:10为流动相,检测波长254mm,分析了火锅底料中的罂粟壳。同时以空白火锅底料及空白底料添加罂粟壳做阴性及阳性对照。实验证实该方法灵敏、准确、可靠。
【Abstract】 Morphine, codeine and papaverine in bottom material of chafing dish were determined by HPLC.The conditions were as follows: C18 column, mobile phase V(CH3OH): V(25 mmol/L KH2PO4)= 90: 10,the flow rate 1 mL/min and the wavelength 254 um. These three compounds were entirely separated. The blank and positive comtrasts were being done simultaneously. The detection limit was 0. 1 mg/L. The experiment showed that this method is sensiuve, accurate and reliable.
【关键词】 高效液相色谱法;
火锅底料;
罂粟壳;
鸦片成分;
【Key words】 high performance liquid chromatography; pericarpium papaveris (Yingsuqiao ); morphine; codeine; papaverine; bottom material of chafing dish;
【Key words】 high performance liquid chromatography; pericarpium papaveris (Yingsuqiao ); morphine; codeine; papaverine; bottom material of chafing dish;
- 【文献出处】 色谱 ,Chinese Journal of Chromatography , 编辑部邮箱 ,1999年04期
- 【分类号】O657.7
- 【被引频次】24
- 【下载频次】296