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反相高效液相色谱法测定酚类化合物的保留指数
Identification of Corrected Retention Indices of Ten Phenols in Reversed-Phase High Performance Liquid Chromatography
【摘要】 以3种ODS柱分别测定了10种酚类化合物的保留值,以烷基甲酮类作为标准参照物计算保留指数及校正保留指数。结果表明,采用校正保留指数系统,可获得比常规保留指数系统更好的精密度和准确性。
【Abstract】 Corrected retention indices in RP-HPLC of ten phenols based on 2-keto-alkanes were examined on three ODS-silica columns. The RI values for phenols showed large variations. However , when the correction was applied, the variations diminished significantly. It is clear that the correction procedure improves the variation of phenols’ RI values obtained from different ODS columns. The procedure is recommended as a method of standardization of HPLC data.
【关键词】 反相高效液相色谱法;
校正保留指数;
保留指数;
【Key words】 high performance liquid chromatography; retention index; corrected retention index;
【Key words】 high performance liquid chromatography; retention index; corrected retention index;
【基金】 国家自然科学基金
- 【文献出处】 色谱 ,CHINESE JOURNAL OF CHROMATOGRAPHY , 编辑部邮箱 ,1999年02期
- 【分类号】O658
- 【被引频次】6
- 【下载频次】101