【摘要】 介绍了高效液相色谱在双酶协同作用酶解制取麦芽低聚糖工艺研究中的应用。以C18柱为分离柱，水作流动相，利用折光检测器来检测麦芽低聚糖产品中的7种糖，同时评估了补加酶量与麦芽低聚糖中麦芽三糖至六糖含量的关系，并测定了二次确认实验中麦芽低聚糖产品中各糖的含量。更多还原

【Abstract】 This paper describes the application of high performance liquid chromatography to the study on the production of maltooligosaccharide by synergistic action of two-enzymes. Seven sugars in products were separated on a column of Nucleosil C18 with water as the mobile phase and determined refractometrically. Meanwhile, for the preparation of maltooligosaccharide, the effect of supplementing rate of the two enzymes on the content of maltooligosaccharide in products is examined in this paper. The optimun technolgical conditions were obtained by using the orthogonal design and the amount of the two enzymes added was determined. Thus more than 70% of M3-M6 maltooligosaccharide in product was obtained.更多还原