【摘要】 研究了壳聚糖涂层在纤维素滤纸上成膜后再固定化猪胰脂肪酶的最佳条件。结果表明，当戊二醛浓度为５％，活化１２ｈ，与ｐＨ７．６的酶的磷酸盐缓冲溶液于室温（１５℃）交联１２ｈ，获得的固定化酶活最高，为０．２６Ｕ／ｃｍ２。固定化酶最适温度４０℃，比游离酶提高了５℃；最适ｐＨ８．５，与游离酶相比，向碱性偏移了０．５个ｐＨ更多还原

【Abstract】 In this paper,the optimum conditions of crosslinking porcine pancreas lipase to chitosan coat carrier were studied.Our results showed that the maximum 0.26U/cm\+2 activity of immobilized lipase was given when the content of glutaraldehyde was 5%,the activitied time was 12h,the temperature and pH of crosslinking reaction was at 15 and 7.6 and the crosslinking reaction time was 12h. The optimum operating temperature and pH of gained immobilized lipase was at 40 and 8.5 respectively.更多还原