【摘要】 对真养产碱杆菌（Ａｌｃａｌｉｇｅｎｅｓｅｕｔｒｏｐｈｕｓ）ＮＣＩＭＢ１１５９９在１０Ｌ发酵罐中利用葡萄糖作为唯一碳源发酵生产聚β－羟基丁酸（ＰＨＢ）的发酵过程进行了研究。在以葡萄糖为唯一碳源的发酵中，分别在缺氧、限氧、限磷情况下及限磷和缺氧情况下对菌体生长及ＰＨＢ积累规律进行了研究。实验表明，氧和磷在菌体生长及ＰＨＢ的积累过程中起着重要作用。缺氧不利于菌体生长及积累ＰＨＢ；在一定程度上限制溶解氧则可促进ＰＨＢ的积累；在供氧充足时，限磷有利于ＰＨＢ大量积累；而限磷和缺氧条件下则抑制菌体生长和ＰＨＢ积累。采用限磷法发酵生产ＰＨＢ，经７２．５ｈ后得到菌体细胞干重为１３８．２ｇ／Ｌ，ＰＨＢ含量为１１１．８ｇ／Ｌ，ＰＨＢ占细胞干重８０．９％。更多还原

【Abstract】 Production of polyhydroxybutyrate(PHB)by Alcaligenes eutrophus NCIMB 11599 in a 10liter fermentor was investigated. Glucose was used as sole carbon source in the fermentation process. In the process, the rules of cell growing and PHB accumulating were examined in four different conditions: oxygen deficiency, oxygen inhibition, phosphorus inhibition, phosphorus inhibition and oxygen deficiency. By contrasting the experimental results, it is found that both phosphorus inhibition and oxygen inhibition can induce the production of PHB. In the phosphorus inhibition process, cell concentration(138.2g/L), PHB concentration(111.8g/L)and PHB content(80.9%)were obtained.更多还原