【摘要】 从Ｃ５７ＢＬ／６Ｊ公鼠脾脏组织抽提大分子ＤＮＡ，进行ＭｂｏＩ部分酶切，低熔点琼脂糖凝胶分离、回收酶切后所需长度的ＤＮＡ片段并与λＧＥＭ○Ｒ－１１ＢａｍＨＩ臂进行连接和λ噬菌体体外包装反应，构建成Ｃ５７公鼠基因组λ噬菌体文库，对实验中遇到的技术问题进行了探讨，比较了两种浓度连接产物的包装效率，并成功地从该文库中筛选到含Ｓｒｙ基因的单克隆更多还原

【Abstract】 High molecular weight genomic DNA was isolated from the spleen tissue of C57BL/6J male mice and was partial digested using restriction endonuclease Mbo I. The DNA fragments of desired size range were eluted from low melting temperature agarose gel electrophoresis and were ligated to the Lambda GEM ○R -11 BamH 1 Arms. Then the li gated DNA was packaged by a Packagene ○R Extract to construct the genomic library of C57 male mouse. Some problems met in experiments were discussed. The comparison was made between the packaging efficiencies of two different concentration of ligation production. The library was screened with Sry probe so a Sry clone (its insert 16×10 3 bp approximately) was gained.更多还原