【摘要】 本文以2／3肝切除（partialhepatectomy，PH）大鼠为模型，探讨了PH后酸性和碱性磷酸酶（acidandalkalinephosphatases，ACP和AKP）、构成性热休克蛋白70／诱导性热休克蛋白68（HSC70／HSP68）、酸性和中性蛋白水解酶在肝再生期间（0～144h）的动态变化。结果显示，在肝切除后的肝再生期间：（1）ACP和AKP均出现两个活性高峰（4和48h、16和96h），在每个活性高峰之后，ACP和AKP活性均有显著下降；（2）HSC70/HSP68在16和96h时出现两个含量高峰，第二个高峰后的最低点（接近于对照）比第一个高峰后的最低点低许多；（3）在2／3肝切除后2－6h时，诱导出一个90kD的中性蛋白水解酶；36h时，诱导出一个27kD的酸性蛋白水解酶。结果提示，ACP和90kD中性蛋白水解酶可能参与激活G0期肝细胞进入G1期；AKP和HSC70／HSP68可能主要在DNA合成、细胞代谢和增殖方面起作用；27kD酸性蛋白水解酶可能在肝细胞再分化和肝组织重建中有一定作用。更多还原

【Abstract】 This article reports changes in acid and alkaline phosphatases (ACP and AKP),conshtutive heat shock protein 70/induced heat shock protein 68 (HSC70/HSP68) andacid and neutal proteinases ddring liver regeneration (0 ~ 144 h) after 2/3 hepatectomy(pwhal hepatectomy, PH). Both ACP and AKP had two achve peaks at 4 and 48 h, 16and 96 h, respectively, which were followed by sighficant decrease. The content ofHSC70/HSP68 also showed two Peaks (16 and 96 h), of which the content after thesecond Peak decnaed more obviously than after the first peak. Moreover a 90 kD neutalproteinase was induced at the time from 2 to 6 h and a 27 kD acid proteinase was inducedat 36 h. The results suggest that the ACP and the 90 kD neuhal proteinase rnayparticipate in activahng hepatocytes from G0-Phase into G1-phase, and are and HSC70/HSP68 may play a role ndnly in DNA synthesis, cellular metabolism and proliferation.Fdriermore 27 kD acid proteinase may be involved in re-differentiation of hepatocytesand reconsmichon of liver tissue.更多还原