【摘要】 建立一种新的制备特异性转移因子的方法。方法：用二硝基氯苯（ＤＮＣＢ）免疫鸡，当观察到迟发型超敏反应发生后，收集鸡蛋制备ＤＮＣＢ高免卵黄透析液，并将其注入小鼠腹腔内，６ ｄ后用 ＤＮＣＢ攻击小鼠并观察其迟发型超敏反应发生的情况。结果：ＤＮＣＢ高免卵黄透析液组、对照卵黄透析液组和生理盐水组的皮肤反应（Ａ值）分别为０．０５２±０．０２２、０．０２８±０．０１４和０．０３２±０．０１２（Ｐ＜０．０５）。结论：高兔卵黄透析液含有某些介导细胞免疫的小分子物质。更多还原

【Abstract】 Purpose: The aim was to find a new method for preparing specific transfer factor (TF). Methods: The hens were immunized with 1-chloro-2, 4-dinitrochlorobenzene (DNCB) and their delayed type hypersensitivity was observed. Hyperimmune egg yolk dialysates to DNCB were prepared and injected into KM mice’s abdominal cavity. Six days later, the mice were challenged with DNCB and delayed type skin hypersensitivity was observed. Results: The skin response (A) of hyperimmune egg yolk dialysates to DNCB group, control egg yolk dialysates group and NS group were 0.052 ± 0. 022, 0. 028 ± 0. 014 and 0. 032 ± 0. 012 respectively (P < 0. 05). Conclusion: Hyperimmune egg yolk dialysates contain certain small molecule substance which can mediate cellular immunoresponse.更多还原