【摘要】 研究一步层析制备高比活抑肽酶新工艺。方法：将化学改性与修饰壳聚糖体，共价偶联配基牛胰蛋白酶，制成固定化酶为抑肽酶亲和吸附剂，直接对黄牛肺提取液一步层析纯化，再超滤脱盐、浓缩、去热原，冻干即抑肽酶。结果：抑肽酶比活５７００ｋｉｕ／ｍｇ，收率２２×１０~4ｋｉｕ／ｋｇ（牛肺）；固定化酶单位活力２５９５０ｋｉｕ／ｇ（干），酶活性回收率５５％，非特异性吸附较小，机械强度高，可反复使用。结论：工艺过程简便，样品比活较高，质量稳定，成本低，适合工业化大生产。更多还原

【Abstract】 Purpose: Research and establish a "one-step" technology for preparation of aprotinin with high purity and high specific activity. Methods: Covalent coupling bovine trypsin with chemically modified chitosan, a kind of immobilized enzyme was obtained and used as the matrix of affinity chromatography for preparation of aprotinin from crude extract of bovine lung directly. To condense the sample and remove the salts and pyrogen, the elute was treated continuously by super- filtration, and finally , lyophilized aprotinin was achieved by freeze-drying. Results: The unit activity of immobilized enzyme matrix was 25 950 kin/Gram (dry weight), and the matrix has less nonspecific absorption, much more times of repeated utilization and hight operation pressure. The specific activity of the final product of aprotinin was as high as 5 700 kin/mg, the recovery rate of aprotinin activity was 55%, and 220 000 kiu aprotinin activity could be obtained from one kilogram bovine lung. Conclusion: The lowcost "one-step" technology for preparation of aprotinin with high purity and high specific activity is suitable for lager scale industrial production.更多还原