【摘要】 通过将ＭＤＲ－１基因（ｍｕｌｔｉｄｒｕｇｒｅｓｉｓｔａｎｃｅｇｅｎｅ）转染入卵巢癌细胞株ＡＯ及３ＡＯ后，探索化疗药物依托伯甙（ｅｔｏｐｏｓｉｄｅ，ＶＰ１６）的药物敏感性，进一步了解卵巢癌细胞株、化疗药物及耐药性之间的关系。方法应用脂质体作为载体将ＭＤＲ－１ｃＤＮＡ导入卵巢癌细胞株ＡＯ及３ＡＯ，转染后４８ｈ加入不同浓度的ＶＰ－１６（１×１０－５ｍｇ／μｌ、１×１０－４ｍｇ／μｌ、１×１０－３ｍｇ／μｌ），加药后在３７℃含５％ＣＯ２的培养箱内培养４８ｈ，然后加入３Ｈ胸苷嘧啶，１８ｈ后测定３Ｈ－胸苷嘧啶掺入量ｃｐｍ值。结果随着ＶＰ－１６浓度的增加，ＡＯ细胞株的３Ｈ－胸苷嘧啶掺入量降低，而带有ＭＤＲ－１基因的ＡＯ细胞株的３Ｈ－胸苷嘧啶掺入量与ＶＰ１６浓度无关，其掺入量在中浓度及高浓度的ＶＰ－１６中均高于ＡＯ细胞株，其差异经ｔ检验，Ｐ＜０．００５及Ｐ＜０．０５。在３ＡＯ细胞株，转染了ＭＤＲ－１基因的细胞３Ｈ－胸苷嘧啶掺入量降低（Ｐ＜０．００１）。随着ＶＰ－１６浓度的增加，３ＡＯ细胞株及带有ＭＤＲ－１基因的３ＡＯ细胞株的３Ｈ－胸苷嘧啶掺入降低，但带有ＭＤＲ１基因的３ＡＯ细胞株的３Ｈ胸苷嘧啶掺入量在中浓度及高?更多还原

【Abstract】 Purpose To explore the effects of multidrug resistance gene (MDR1) transfection into human ovarian cancer cell lines AO and 3AO on sensitivity to chemotherapeutic agent Etoposide(VP16) in order to know the relationship of cancer cell line,chemotherapeutic agent and drug resistance . Methods (1)MDR1 cDNA was introduced into AO and 3AO cells using lipofection.(2)Cellular culture:After transgene,48 hours later,antineoplastic VP16 was added to the cell culture.After an additional 48 hours of cell culture in 5% CO 2 at 37℃, 3Hthymidine was added.18 hours later, 3 Hthymidine uptake was determined. Results Following the concentration of VP 16 increasing, AO cells showed a dose dependent decrease in 3 H thymidine uptake.But AOMDR 1 cells showed a dose dependent decrease in 3 H thymidine uptake. 3H thymidine uptake was high in AO MDR 1 cells than AO cells at mo-derate and high concentration of VP 16( P <0.005, P <0.05).In 3AO cell lines,3AO MDR 1 cells showed a decrease in 3H thymidine uptake( P <0.001).Following the concentration of VP 16 increasing,3AO cells and 3AO MDR 1 cells showed a dosedependent decrease in 3H thymidine uptake.But 3H thymidine uptake was higher in 3AO MDR 1 cells than 3AO cells at moderate and high concentration of VP 16( P <0.05, P <0.001). Conclusions AO or 3AO cells transfected MDR 1cDNA may appear cells biologic activated change.MDR 1 gene expression,cell’s type,chemotherapeutic agent and the dose of agent may influence chemosensitivity.更多还原