【摘要】 目的　研究戈米辛J(gomisinJ)对氧自由基的作用。方法　采用黄嘌呤/黄嘌呤氧化酶鲁米诺体系和邻苯三酚自氧化体系产生的化学发光检测超氧阴离子自由基,采用Fenton发光体系检测羟自由基。结果　戈米辛J能抑制3种体系的化学发光,对以上3种体系的IC50、IC20及IC50(95%可信限)依次分别为237.7(155.5～363.3)、100.8(88.1～115.3)和1.86(1.60～2.16)μmol/L。结论　戈米辛J具有清除氧自由基的作用,对羟自由基作用尤强更多还原

【Abstract】 To study the effects of gomisin J on oxygen free radicals. Methods Xanthine/xanthine oxidase*.luminol chemiluminescence system,pyrogallol autoxidation chemiluminescence system,Fenton reaction phelanthroline chemiluminescence system were used to measure oxygen free radicals. Results Besides a O -· 2 scavenger with the IC 50 (95%CL) of 237.7 (155.5～363.3) μmol/L in xanthine/xanthine oxidase system and wity the IC 20 (95% CL) of 100.8 (88.1～115.3) μmol/L,gomisin J scavenged ·OH efficiently in Fenton like system,the IC 50 (95% CL) was 1.86 (1.60～2.16) μmol/L Results Besides a O -· 2 scavenger with the IC 50 (95%CL) of 237.7 (155.5～363.3) μmol/L in xanthine/xanthine oxidase system and wity the IC 20 (95% CL) of 100.8 (88.1～115.3) μmol/L,gomisin J scavenged ·OH efficiently in Fenton like system,the IC 50 (95% CL) was 1.86 (1.60～2.16) μmol/L Conclusions Gomisin J is a superoxide anion scavenger and a strong hydroxyl radical scavenger.更多还原