【摘要】 荧光显微镜观察及ＥＬＩＳＡ分析表明１０μｍｏｌ／Ｌ过氧化亚硝基可诱导原代培养的大鼠小脑颗粒神经元凋亡。应用ＥＳＲ自旋标记技术研究了这一过程中细胞膜生物物理特性的变化，结果表明神经细胞经过氧化亚硝基处理后，细胞膜表层及深层流动性均显著下降，膜蛋白巯基强弱固定化比值增加。维生素Ｃ、维生素Ｅ的衍生物Ｌ抗坏血酸２［３，４二氢２，５，７，８四甲基２（４，８，１２三甲基十三烷基）２Ｈ１苯并吡喃６磷酸酯］钾盐（ＥＰＣＫ１）能减轻过氧化亚硝基对细胞的氧化损伤、防止细胞凋亡。更多还原

【Abstract】 The cytotoxic effect of peroxynitrite on cerebellar granule neurons was studied. Exposure of cerebellar granule neurons to 10 μmol/L peroxynitrite triggered apoptosis in vitro, which was confirmed by both morphological(nuclear morphology observed by fluorescence microscopy) and biochemical evidence(DNA fragmentation detected by ELISA). Using ESR spin labeling technique, the alteration of biophysical characteristics of neuronal cell membrane during the apoptotic process was studied. The results indicate that after treatment with peroxynitrite, the fluidity of both the surface layer and the deep layer of the neuronal cell membrane decreased markedly, and the S/W ratio of the membrane protein thiol groups increased significantly. Pre treating cerebellar granule neurons with antioxidant EPC K1, a novel water soluble derivative of vitamin C and vitamin E, alleviated the oxidative injury and prevented cells from apoptosis.更多还原