【摘要】 以水囊引产５ 、６ 、７、８ 个月人胚胎鼻咽组织总ＲＮＡ 逆转录标记ｃＤＮＡ 探针， 与代表５８８ 个基因的ＡｔｌａｓＴＭｃＤＮＡ阵列进行杂交， 观察了这些基因在不同发育时期内的表达差异。结果发现与细胞分裂增殖及细胞生长相关的基因明显高表达， 不同胎龄存在多个表达水平不同的基因及同一基因在不同时期表达水平也不一样， 如早期生长反应蛋白１（ｅａｒｌｙ ｇｒｏｗｔｈ ｒｅｓｐｏｎｓｅ ｐｒｏｔｅｉｎ １）基因ＥＧＲＰ１ 在５ 、６ 个月高表达而７ 、８ 个月低表达，ＲＴＰＣＲ 也证实该结果。结果表明： ＥＧＲＰ１ 等基因表达在人胚鼻咽组织发育中存在明显的时间依赖性特征；ｃＤＮＡ阵列在研究组织内多个基因的表达谱差异方面提供了十分重要的研究工具。更多还原

【Abstract】 To study differentially expressed genes in nasopharynx tissues of embryo during development, and to observe the gene changes, total RNAs were respectively extracted from the nasopharynx tissue which came from fetuses of 5, 6, 7, and 8 months, probes were yielded by reverse transcription and were used to hybridize with the Atlas TM human cDNA expression arrays. The results showed that the genes expression profile were distinctly different and different expression levels were found in the same gene during development. Results indicated that gene expression pattern had a character of time dependence, such as early growth response protein 1; and the cDNA expression array provided a powerful method for studying gene expression profile in a large range of genes.更多还原