【摘要】 观察了脓毒血症大鼠心肌ＩＩ型ＰＬＡ２ 活性、蛋白质含量及其ｍ ＲＮＡ 的变化。结果发现， 脓毒血症早期与晚期心肌ＩＩ型ＰＬＡ２ 活性较对照组分别降低２５ ．０ ％ （Ｐ ＜ ０ ．０５）及增高４７．６ ％ （Ｐ ＜ ０ ．０１），ＩＩ型ＰＬＡ２ 蛋白质含量分别降低２７．０％ 及增高４８ ．０ ％（ 均Ｐ ＜ ０ ．０１）； 心肌ＩＩ型ＰＬＡ２ ｍ ＲＮＡ合成率与含量呈现类似的双相变化， 在脓毒血症早、晚期ｍＲＮＡ 合成率分别降低４５．０％ 和升高７０．０ ％ （均Ｐ ＜ ０ ．０１），ｍＲＮＡ含量分别降低３４．１ ％ 和增加１５７ ．０％ （均Ｐ＜ ０ ．０１） 。脓毒血症早、晚期心脏ＩＩ型ＰＬＡ２ ｍ ＲＮＡ半衰期无显著变化（Ｐ ＞ ０．０５） 。实验结果表明大鼠脓毒血症发生过程中心肌ＩＩ型ＰＬＡ２ 活性呈现出先下降后升高的变化， 这一变化受其ｍＲＮＡ 转录水平的调节。更多还原

【Abstract】 Changes of activity and content of myocardial group II phospholipase A 2 and its mRNA transcription and stability during rat sepsis were investigated. Results showed that, compared with control group, myocardial group II phospholipase A 2 activity in early and late sepsis decreased by 25.0%( P < 0.05) and increased by 47.6%( P < 0.01), respectively; group II phospholipase A 2 protein concentration reduced by 27.0% and augmented by 48.0%( P < 0.01), respectively. Myocardial group II phospholipase A 2 mRNA transcription rate and content showed similar two phases changes. The mRNA transcription rate during early and late sepsis decreased by 45.0% and increased by 70.0%( P < 0.01), respectively. The mRNA content decreased by 34.1% in early sepsis and increased by 157.0% in late sepsis( P < 0.01), respectively. The half life of group II phospholipase A 2 mRNA remained unchanged notably during early and late stage of sepsis. These data suggest that myocardial group II phospholipase A 2 activity decreased in early stage of sepsis and increased in its late stage, and these changes were regulated transcriptionally.更多还原