【摘要】 将脂肪酶固定在ｐＨ玻璃电极上，制成甘油三酯酶传感器．对传感器的制作方法和响应性能进行了研究．在３７℃，选择ＴｒｉｓＨＣｌ缓冲溶液（ｐＨ８５）为测量介质，所制传感器对甘油三酯的响应线性范围为３０９×１０－６～１９１×１０－３ｍｏｌ／Ｌ，响应斜率为３２７ｍＶ／ｐＣ，响应时间为５～１０ｍｉｎ，使用寿命可达２５ｄ．用研制的传感器测定了人和兔血清中的甘油三酯含量，结果满意．更多还原

【Abstract】 A triglyceride enzyme sensor has been prepared by immobilizing lipase on pH glass electrode. The immobilized methods of lipase and the response characteristics of the sensor were studied.The linear range of detection is 3 09×10 -6 ～ 1 91×10 -3 mol/L for triglyceride with a slope of 32 7 mV/pC in the pH 8 5 Tris HCl buffer solution at 37℃. The response time is 5～10 min. The sensor is relatively stable for 25 days.The sensor has been applied to the determination of triglyceride in serum with satisfactory results.更多还原