【摘要】 以大熊猫脑为材料，经提取、热处理、ＰｈｅｎｙｌＳｅｐｈａｒｏｓｅＣＬ４Ｂ疏水柱和快速液相分子筛层析，分离纯化得到ＣａＭ．经ＳＤＳＰＡＧＥ、ＰＡＧＥ和ＩＥＦ鉴定，得到的ＣａＭ为一条带．经测定，大熊猫脑ＣａＭ的分子质量为１９ｋｕ，等电点为３８．酶活性实验表明大熊猫脑ＣａＭ对牛心磷酸二酯酶有激活作用．氨基酸组成分析结果与其他来源ＣａＭ相近．更多还原

【Abstract】 Giant panda brain CaM was purified by Phenyl Sepharose CL 4B hydrophobic chromatography and FPLC. The results of SDS PAGE, PAGE and IEF indicate that the protein has been purified . The molecular weight and PI of CaM are 19 ku and 3 8, respectively. Giant panda brain CaM shows calcium ion effect in SDS PAGE and PAGE. It can activate bovine heart PDE. The result of amino acid composition of giant panda brain CaM is similar to CaMs from other sources.更多还原