【摘要】 以人胚胎ｍ Ｒ Ｎ Ａ 为模板， 采用 Ｒ Ｔ Ｐ Ｃ Ｒ 法得到了人补体调节蛋白膜辅蛋白（ Ｍ Ｃ Ｐ） 的一种ｃ Ｄ Ｎ Ａ 全基因，序列分析结果表明， 所获得的 Ｍ Ｃ Ｐｃ Ｄ Ｎ Ａ 为文献报道中１０ 种同种型中的一种， 属 Ｍ Ｃ Ｐ Ｃ２ 型， 该ｃ Ｄ Ｎ Ａ 含一编码３６９ 个氨基酸的阅读框架， 其中的 Ｓ Ｔ Ｐ 区含１４ 个氨基酸， 由 Ｓ Ｔ Ｐ Ｃ 编码， 胞浆尾区含２３ 个氨基酸， 为 Ｃ Ｙ Ｔ２ ，未发现有东西方人种之间的核苷酸的差异更多还原

【Abstract】 MCP is an important membrane protein for protecting host cells from damage by complement because of its wide tissue distribution and cofactor activity . A human MCP cDNA containing full encoding region was obtained by RT PCR from Chinese human embryo. The target gene was cloned and sequenced. The data indicated that there are forty five bases deletion compared with the counterparts previouly reported,which leads to the deletion of fifteen amino acid residues. The PCR product including 369aa open reading frame was one of the ten isoforms which had been reported before and it was named MCP C 2.更多还原