节点文献
花粉管导入法培育抗除草剂的转基因水稻
TRANSGENIC HERBICIDE-RESISTANT RICE PRODUCED WITH SPERMARY INTRODUCTION
【摘要】 以含Bar 基因的pAHC20 为供体DNA,上农香糯植株的稻穗为受体材料,采用花粉管导入法进行基因转化,经除草剂筛选,获得抗除草剂的转基因上农香糯后代;除草剂试验表明,转基因水稻种子的萌发和生长对除草剂的抗性比未转化的上农香糯强,差异达极显著。
【Abstract】 Using pAHC20 with Bar gene as donor DNA, the flowers of Shangnong aromatic glutinous rice as recipient were introduced herbicide-resistant gene with pollen tube way. After chosen with herbicides, the seeds of transgenic rice were obtained. The herbicide experiments showed that the herbicide-resistance of transgenic rice seeds germinating and growing were stronger very significantly than nontransformation Shangnong aromatic glutinous rice.
【关键词】 水稻;
抗除草剂;
Bar基因;
花粉管导入;
【Key words】 Oryza sativa; Herbicide-resistance; Bar gene; Introduction with pollen tube way;
【Key words】 Oryza sativa; Herbicide-resistance; Bar gene; Introduction with pollen tube way;
【基金】 上海市教委“曙光计划”资助!(96SG19)
- 【文献出处】 上海农学院学报 ,JOURNAL OF SHANGHAI AGRICULTURAL COLLEGE , 编辑部邮箱 ,1999年04期
- 【分类号】S511.034
- 【被引频次】24
- 【下载频次】191