【摘要】 采用人PBMC为实验体系,用本中心建立的生物素链霉亲和素免疫细胞化学法CD2+ 细胞增长试验对IL2 和IFNγ等9 种免疫调节药物进行活性鉴定,并用流式细胞仪加以验证。结果表明,IL2 、IFNγ和TNF 等重组细胞因子制剂均对人PBMC表达CD2 抗原有强促进作用,CD2+ 细胞增长率在100 % 左右,LMZ、转移因子、核酪以及胸腺肽等生物制品的作用稍逊,CD2+ 细胞增长率在50 % ～70% ,不同厂家生产的胸腺肽制剂对CD2 抗原表达显示不同程度的促进作用,地塞米松则有抑制CD2 抗原表达的作用,据此认为CD2+ 细胞增长试验在筛选和鉴定免疫制剂中具有良好的应用价值更多还原

【Abstract】 The activities of nine immunomodulators were determined by proliferation test of the CD2 + cells by which some of them determined by FACS The results showed that recombinant cytokines IL 2,IFN γ and TNF α could promote the expression of CD2 remarkably The proliferation rates of CD2 + cells were approximately 100%,while the effects of LMZ,transfer factor and thymosin were less significant in which the proliferation rates of CD2 + cells appeared to be 50% to 70% Effects of thymosins from various manufacturers were different,Dexamethasone inhibited the expression of CD2 on PBMC It is suggested that the proliferation test of CD2 + cells is of value in screening and evaluating immunomodulators更多还原