【摘要】 目的研究ＴＧＦ在深Ⅱ度烧伤愈合中表达变化，为临床合理应用外源性ＴＧＦ提高疗效，缩短疗程提供实验依据。方法在ＳＤ大鼠背部埋入海绵，于热烫伤深Ⅱ度后１、３、５、７、１０ｄ分别取渗出液细胞，采用ＲＴ－ＰＣＲ和地高辛原位杂交方法检测渗出液细胞中ＴＧＦβ1；的ｍＲＮＡ表达量的变化；取人深Ⅱ度烧伤后５、７、１２ｄ手术得到烧伤皮肤及其临近的正常皮肤，采用ＲＴ－ＰＣＲ和免疫组化方法各时相烧伤皮肤及其临近的正常皮肤ＴＧＦβ１的ｍＲＮＡ与蛋白质的表达变化。结果在大鼠深Ⅱ度烧伤愈合时渗出液细胞中ＴＧＦβ１在５～１４ｄ有高表达。人深Ⅱ度烧伤后烧伤皮肤ＴＧＦβ１的ｍＲＮＡ和蛋白质在７ｄ出现表达峰，在其临近的正常皮肤中ＴＧＦβ1表达稳定。结论通过该研究说明在深Ⅱ度烧伤早期ＴＧＦβ１较高表达促进愈合，为临床合理应用外源性ＴＧＦβ１提高疗效，缩短疗程提供实验依据。更多还原

【Abstract】 Objective To study the change of TGF expression in the healing process ofdeep Ⅱ burn, and provide evidence to the clinical application using exogenous TGF in orderto enhance the efficacy and shorten the therapy period. Methods Sponge was implantedinto the back of SD rat, and the exuded cells were obtained at day 1,3,5,7,and 10 respectivelyafter deep Ⅱburn caused by heating. RT- PCR and DIG in situ hybridization were used todetect the change of amount of TGFβl mRNA expression. Similarly, samples of both deep Ⅱburned skin and the adjacent normal skin obtained from patients were carried out RT - PCRand immunol histochemistry analysis. Results The expression of TGFβ1 in exudates of deepⅡ burned rat was of highest level at day 5~10, while the deep 11 burned skin obtained frompatients has the highest expression level of TGFβl at day 7 in both mRNA and proteinlevel. Conclusion The above results indicate that the high expression level of TGFβl at theearly stage of deep Ⅱ burning might promots the healing process, thus provide evidence tothe clinical application using exogenous TGF in order to enhance the efficacy and shortenthe therapy period.更多还原