【摘要】 采用酶免疫（ＥＩＡ）和反转录聚合酶链反应（ＲＴ－ＰＣＲ）技术对不同临床型ＨＣＶ感染患者的血清标本进行ＨＣＶ 分型。结果显示，各临床型ＨＣＶ 感染的型别构成无明显差异，主要为基因Ⅱ型（９３．７％ ）、少量基因Ⅲ型（２．７％ ）和Ⅱ＋ Ⅲ混合型（３．６％ ），未见基因Ⅰ、Ⅳ型。基因分型与血清学分型总符合率为９７．９６％ （４８／４９），血清学分型率与ＨＣＶ 抗－ＣＳ／ＣＯ值呈正相关；ＨＣＶ－ＲＮＡ阳性者９３．７５％可基因分型，但仅有５２．０８％ 可血清学分型，ＨＣＶ－ＲＮＡ 阴性而抗ＨＣＶ 阳性者６８．９７％ 的可血清学分型。表明该地区ＨＣＶ感染主要为基因Ⅱ型，少许基因Ⅲ型和Ⅱ＋ Ⅲ混合型。基因、血清学分型结果具有良好的一致性，两种分型有一定互补作用。更多还原

【Abstract】 The serum of different clinical patients with hepatitis C virus have been classified by EIA of antigens and RT PCR techaique.The results show that there is no obvious difference between different clinical patients infected with hepatitis C virus and patients with hepatitis C.Most of them are genic type Ⅱ(93.7%),others are genic type Ⅲ(2.7%) and mixed type Ⅱ and type Ⅲ(3.6%),Genic type Ⅰ and type Ⅳ are never seen.The rate of genic type and serum type is 97.96%.Serum type and HCV anti C S/Co are positive relations.The gence of(93.75%)patients with positive HCV RNA may classified and serum of 52.08% may only classified.The serum of(68.97%)patients with negative HCV RNA but positive HCV may classified.It shows that in this area the patients with HCV are mainly infected by genic type Ⅱ,only a few patients are infected by genic type Ⅲ and mixed type Ⅱ and type Ⅲ.The result of the classification of gence and serum are fine consistency.The methods of serum classification and genic classification have certain complentary action.更多还原