【摘要】 从成熟番茄果实中分离mRNA，以Oligo（dT）为引物在AMV逆转录酶作用下合成ACC2cDNA第一链，以cDNA为模板通过PCR扩增ACC2基因，获得1.45kb的扩增片段，将此片段克隆到BS质粒的EcoRV位点上，对插入片段两端进行部分序列分析，随后，将此片段以反方向插入双元载体pBI151的35S启动子和NOS终止子之间，通过三条交配法将携带反义ACC2基因的中间表达载体pBA7转入根癌农杆菌LBA4404，得到了完整的Ti质粒表达载体系统。更多还原

【Abstract】 The first strand of cDNA was synthesized from mRNA of the ripe tomatofruit by using AMV reverse transcriptase and oligo(dT) primer. A 1 .45kb specific fragment was obtained through PCR amplification of ACC2 gene from cDNA, and clonedinto BS plasmid digested with EcoRV. The sequence of both ends of the insert was identical to the results reported by Rottmann et all. The ACC2 gene sequence was theninserted in reverse orientation between the CaMV 355 promoter and NOS tendnator intothe binary vector pBI151.更多还原