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平行检测血清和尿液中循环抗原诊断日本血吸虫病的研究

STUDIES ON THE DIAGNOSIS OF SCHISTOSOMIASIS JAPONICA BY PARALLEL

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【作者】 刘晓明王之怀王勇尹晓梅汪昊季虹吴维铎

【Author】 Liu Xiaoming, Wang Zhihuai, Wang Yong, et al. Anhui Provieial Institute of Schistosomiasis (Wuhu 241000)

【机构】 安徽省血吸虫病防治研究所安徽省血吸虫病防治研究所 芜湖市 241000芜湖市 241000

【摘要】 应用McAb-Dot-ELISA平行检测了人宿主的血清和尿样(浓缩20倍)中日本血吸虫肠相关趋阴极循环抗原(CCA)。81例急性血吸虫病患者的血清和尿样的阳性率分别为96.30%和66.67%。而血清和尿样组合检测的阳性检出率提高至100%。109例慢性血吸虫病患者的血清和尿样的阳性检出率分别为77.06%和10.09%,组合检测的阳性检出率没有提高。100份健康人的血清和尿样的假阳性率均为0%。53例华枝睾吸虫病患者的血清和尿样的交叉反应率分别为9.43%和0%。48例钩虫病患者的血清和尿样的交叉反应率均为0%。

【Abstract】 The levels of Schistosoma circulating cathodic antigen (CCA) in serum and urine (given a 20 - fold concentration ) were detected parallel by McAb - Dot - ELISA. CCA in serum and urine were positive in 96.30 % and 66.67%, respectiuely, of 81 patients with acute schistosomiasis, while the sensitivity was increased to 100 % by parallel testing. The sensitivity in serum and urine of 109 patients with chronic were positive in 77.06 % and 10.09%, respectively. Both the false positive rates in serum and urine of 100 healthy controls were 0%. The cross reaction rates in serum and urine of 53 cases with clonorchiaisis were 9.43 % and 0 % respectively. Both cross- reactions in serum and urine of 48 patients with ancylostoniasis were 0 %.

【基金】 本研究获得世界银行贷款中国血吸虫病控制项目MOPH/TDR,联合科研管理委员会(JRMC)资助
  • 【文献出处】 热带病与寄生虫学 ,Journal of Tropical Diseases and Parasitology , 编辑部邮箱 ,1999年01期
  • 【分类号】R446.6
  • 【下载频次】15
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