【摘要】 报道以青岛野生型龙须菜（ｗｔ）、黄色突变体（ｙｅ１００）、４株绿色突变体（ｇｒ１８０、ｇｒ１８４、ｇｒ１８６、ｇｒ１８８）、委内瑞拉野生型龙须菜（ｌｖ）、南非野生型龙须菜（ｓａ）及真江蓠（ｇａ）为材料，用蔗糖密度梯度离心法得到完整的藻胆体。用５４０ｎｍ激发，野生型的荧光发射峰位于５７８ｎｍ和６７０ｎｍ，相对荧光强度ＦＡＰＢ／ＦＰＥ的比值为１．５０。几种材料的ＰＥ和ＡＰＢ荧光发射峰没有明显区别（不超过２ｎｍ），但两峰荧光强度比值显著差异。结果显示ｌｖ的ＦＡＰＢ／ＦＰＥ比值最低，只有１．２０，ｇｒ１８６的比值最高，为５．６７，野生型中ｓａ也比较高，为４．７９。用６７０ｎｍ激发，野生型龙须菜的荧光社发光谱有５个峰，分别为４９９ｎｍ、５４０ｎｍ、５６８ｎｍ、６１４ｎｍ、６５０ｎｍ，与其吸收光谱一一对应。野生型和突变体、不同产地龙须菜及真江蓠的荧光发射峰的波长都与其吸收光谱相对应。更多还原

【Abstract】 A comparison of different room temperature florescent emission spectra of phycobilisome was made in the present paper. The emission peaks of 540nm of wt were at 578nm and 670 nm, and the relative fluorescence ratio of F APB /F PE was 1.50. Compared with wt, the room temperature fluorescent emission spectra of these materials were basically the same (within 2nm), but the fluorescence intensity ratio varied remarkably. The ratio F APB /F PE of lv was the lowest, only 1.2, and that of gr186 was the highest, 5.67. The emission peaks of 670nm of wt were at 499nm, 540nm, 568nm, 614nm and 650nm, respectively. Of all the materials, including wt and mutants, wt from different habitats of Gracilaria lemaneiformis , and G. asiatica, the wavelengths of emission peaks were corresponding to those of their absorption peaks, respectively.更多还原