【摘要】 构建了两个含高赖氨酸蛋白质基因cDNA的表达载体，用基因枪法将其导入玉米不同杂交组合的胚性愈伤组织，经潮霉素抗性筛选，得到了可育的再生植株，经PCR扩增分析、点杂交、及Southern杂交检测，表明该基因已整合到玉米的基因组中。测定13株T1代种子中赖氨酸的含量，其中有3株赖氨酸含量提高10％以上。更多还原

【Abstract】 Two expression vector contain cDNA encoding a Lysine-rich protein had been contrycted. Embro and embryogenic maize callus culutres from four hybrid lines were transformed by microprojectile bombardment with the two construct. Regenerated fertile transformed plants were obtained by selecting for hygromycin resistance. Genomic PCR, Dot blotting, Southern blotting analysis showed that the construct was integrated into the maize genome with different copy number Fertiled seeds were obtained from some transgenic plants. The content of Lysine in plant seeds of l3 transgenic plant were analysised. The percent of Lysine of three transgenic plant was increased more than 10 %.更多还原