【摘要】 以多寄主可移动载体质粒pSUP1011为基础载体，构建了绿色荧光蛋白（GFP）标记的重级质粒，通过细菌接合，将重组质粒转移到嗜水气单胞菌J－l中。经紫外光检测和SDS－PAGE电泳分析，证实重组质粒在该富中可表达GFP。在无选择压力的培养条件下，24h后，重组质粒的稳定率在72．2％；36h后，稳定率在50％。显示GFP作为报告基因在嗜水气单胞菌致病机理研究的应用前景。更多还原

【Abstract】 A recombinant plasmid pSUP1011(GFP) containing GFP marker was conwicted with the mobilizable vector plasmid pSUP1011, and transferred into Acmmonas hydrophila (Ah) strain J-l by bacterial conjugation. It was demonstrated that GFP was expressed in the transferred bacteria by detection of UV illumination and SDS-PAGE electrophoresis analysis. Under nonselective condition, the stability of recombinant plasmid was 72.2 % after 24h, and 50 % after 36 h. It showed that GFP may be of application potential in investigating pathogenic mechanism of Ah as a reporter gene.更多还原