【摘要】 本研究通过油菜与萝卜属间杂种（ACR）为亲本材料，分别与芸苔B.campestris和甘蓝B.oleracea杂交，属种间杂种（AACR，ACCR）再与甘蓝型油菜品种杂交，以获得稳定的转基因属种间杂种材料。结果指出，属间杂种与芸苔B．campestris杂交比与及甘蓝B.oleracea杂交更易获得后代。这些杂种后代分别与甘蓝型油菜回交也表现了这种趋势。细胞学分析指出，属种间杂交由于异源染体的存在，在花粉母细胞减数分裂期，染色体分裂，配对行为极为复杂，不但有二价体，还有单价体，多价体（三价体，四价体，五价体）出现。减数分裂后期出现了三核、多核现象，也存在染色体缺失，落后以及染色体桥等，研究指出减数分裂期遗传障碍是属种间不育的主要原因之一。更多还原

【Abstract】 In order to obtain a hybrid of transfer gene, the investigation was carried out that the hybrids (ACR) betWeen B. napus and R. sativus were crossed with B. earn~ or B. olcracca, then with B. napus. The results indicated that the crossing betWeen hybrids and R eampestris was easy to obtain back crossing hybrids than that with B. olcracca. The chromosome configurations of PMCs in MI of meiose were complex resulting from the A. C and R genome associations and recombination. In MI phase ofmeiose univalents and multivalents were found, expect bivalent in Mll triade and hexade, chromatiafragmente were also observed. This resulted in a high sterility or only partial fertility of the hybrids.更多还原